dextran hydrodynamic radius

Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. 2013;3(5):3588605. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Biomaterials, 22, 1891-1898. Curley SA. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Front Microbiol. ; Yevlampieva N.P. Ortega, A.; Garca de la Torre, J. Smith, David R.; Raymonda, John W. (1972). (2013). In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Int. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). CAS Materiaux 1-5. Progress in Polymer Science, 30, 38-70. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. 2009;8(2):1739. 2012;3(1):208. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Food Sci Biotechnol. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Biophysical Journal, 87, 4259-4270. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. J. Braz. Radiofrequency ablation of malignant liver tumors. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Intrinsic viscosity-molecular weight relationship for chitosan. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Webof overall macromolecular geometry and hydrodynamic draining. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Recent Pat. Cookies policy. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. (2008). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. ; Ghosh, T.K. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. 1984;17(6):11703. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. MeSH terms Colloid Polym. Structure Properties of Dextran. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. 8. With FITC-dextran 20 K, cumulative Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. The mechanism of the dextran-induced red blood cell Aggregation. Harding, Stephen E. (1997). A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). ; Talsma, H.; Hennink, W.E. This conjecture found to be correct. 2. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). J. Phys. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Format), Citation-(BibTeX Figure5 shows the cumulative amount of the FITC-dextran (M.W. AATEX. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. 2003;10:33847. Sachdeva V, Banga AK. Determination of intrinsic viscosities of macromolecules and nanoparticles. 2015;32(5):170413. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. If there are particles in the sample, the incident laser light gets scattered in all directions. Carbohydrate Polymers, 68, 477-488. Theory, Estimation, Experiment, and Data. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Alternatives to animal testing: a review. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) Formation of dextran hydrogels by crystallization. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Colloid and Polymer Science, 286, 1223-1231. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Walkenhorst, S.; Olivier, R. (2002). Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. 2009;301(8):60913. Carbohydrate Polymers, 38, 267-271. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. CAS The Intrinsic Viscosity of Biological Macromolecules. Expert Rev Med Devices. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Advances in Cosmetic Surgery. Google Scholar. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in ; De Jong, S.J. sharing sensitive information, make sure youre on a federal Terms and Conditions, Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. 113, 81-86. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. (2004). Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. (2008). ; Fran, H.P. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Drug Deliv. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. Progress in Biophysical Molecules Biological, 68, 207-262. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Huggins, Maurice L. (1942). Transdermal drug delivery: overcoming the skins barrier function. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Manage cookies/Do not sell my data we use in the preference centre. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Harding, Stephen E. (2005). If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Biomaterials. Charge: Anionic. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Comparison of single-point and dilution procedures. Increased TEWL values are evidence of micropores formation. California Privacy Statement, Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Drug Deliv Syst. (2008). In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. 2000;3(9):31826. (1999). 2012;87(6):1728. Garcia de la Torre, J.; Carrasco, B. ; Hogan, M.P. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Dextran Fractions. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Prausnitz MR, Langer R. Transdermal drug delivery. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. (1937). Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. II. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Springer Editions. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. ; Rjumtsev, E.I. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. 43-1, 115-147. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. 2012;9(2):14754. Kumar SV, Tarun P, Kumar TA. Pharmacol Pharm. European Polymer Journal, 37, 619-622. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Indo AM J Pharm. B 113, 8020-8025. Hydrodynamic radii ( RH) were determined by dynamic light scattering. Herwadkar A, Banga AK. Critical parameters for gel permeation (1990). 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Dextran produced from Weissella cibaria JAG8 was purified and characterized. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. TEWL measurements were performed before and 30min after microporation. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Molecular weight (M v) and hydrodynamic properties can be calculated from there. The human and pig skin permeability of FITC-dextran (M.W. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Masuelli, M. A. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. 2019;9(3-S):9726. Pure Appl. where tr is the column retention time in minutes (2 of 0.9792). Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50].